- 1 What is solid phase extraction method?
- 2 What is a solid matrix?
- 3 How does the stationary phase of solid phase extraction work?
What is solid phase extraction method?
Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g. HPLC, GC, TLC). In SPE, one or more analytes from a liquid sample are isolated by extracting, partitioning, and/or adsorbing onto a solid stationary phase.
What is the solid phase in chromatography?
Chromatography is a separation process involving two phases, one stationary and the other mobile. Typically, the stationary phase is a porous solid (e.g., glass, silica, or alumina) that is packed into a glass or metal tube or that constitutes the walls of an open-tube capillary.
What is matrix solid phase dispersion?
Matrix solid phase dispersion (MSPD) is a technique widely used in sample preparation for the extraction of chemical constituents from fruits and plants, as well as for the extraction of organic contaminants from food and environmental samples.
What is dispersive SPE?
QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) dispersive SPE, is the number one used sample preparation approach in pesticide residue analysis. It eliminates complex liquid extraction methods and extends the range of recovered pesticides.
What is dispersive liquid liquid microextraction?
Dispersive liquid-liquid microextraction (DLLME) is an extraction technique developed within the last decade, which involves the dispersion of fine droplets of extraction solvent in an aqueous sample. Dairy products present a complex sample matrix and the removal of interfering matrix components can prove troublesome.
What does solid phase mean?
Solid-phase meaning (physics) One of the three fundamental structural phases of matter in which the cohesive force of matter is strong enough to keep the molecules or atoms in the given positions, restraining the thermal mobility. noun.
What is a solid matrix?
solid matrix definition: An assembly of interconnected solid mineral grains by voids Definition of solid matrix: An assembly of interconnected solid mineral grains surrounded by voids . ” />
What is solid phase extraction used for?
Solid phase extractions (SPEs) are routinely used in the sample preparation for quantifying analytes in biological fluids such as plasma and urine. This technique allows both removal of interfering biological matrix components and enhancing the concentrations of analytes in LC/MS samples.
What is QuEChERS extraction?
QuEChERS is a solid phase extraction method for detection of pesticide residues in food. The name is a portmanteau word formed from “quick, easy, cheap, effective, rugged, and safe”.
What is a dispersive liquid?
Abstract. Dispersive liquid-liquid microextraction (DLLME) is an extraction technique developed within the last decade, which involves the dispersion of fine droplets of extraction solvent in an aqueous sample.
What is microextraction method?
Microextraction is defined as an extraction technique where the volume of the extracting phase is very small in relation to the volume of the sample, and extraction of analytes is not exhaustive. From: Journal of Chromatography A, 2000.
What is dispersive solid phase extraction ( D-SPE )?
QuEChERS Dispersive Solid Phase Extraction. Dispersive solid phase extraction (d-SPE) analysis is a simple and straightforward sample preparation technique suitable for a wide variety of food and agricultural products. One specific method which has become popular is the QuEChERS technique which was created to facilitate the rapid screening…
How does the stationary phase of solid phase extraction work?
As the sample passes through the stationary phase, the polar analytes in the sample will interact and retain on the polar sorbent while the solvent, and other non-polar impurities pass through the cartridge. After the sample is loaded, the cartridge is washed with a non-polar solvent to remove further impurities.
What do you need to know about solid phase microextraction?
Solid-phase microextraction (SPME), is a solid phase extraction technique that involves the use of a fiber coated with an extracting phase, that can be a liquid ( polymer) or a solid ( sorbent ), which extracts different kinds of analytes (including both volatile and non-volatile) from different kinds of media, that can be in liquid or gas phase.
How is DSPE used to extract analytes from the matrix?
To achieve analytical success, it is necessary to develop thorough clean-up procedures to extract analytes from the matrix. Dispersive solid phase extraction (DSPE) has been used as a pretreatment technique for the analysis of several compounds.