What is enzyme alpha-glucosidase?

Glucosidase enzymes catalyze hydrolysis of starch to simple sugars. In humans, these enzymes aid digestion of dietary carbohydrates and starches to produce glucose for intestinal absorption, which in turn, leads to increase in blood glucose levels.

What is the structure of acid alpha-glucosidase?

GAA is synthesized as a 110 kDa glycoprotein, which is targeted to the lysosome via the mannose-6-phosphate receptor and undergoes in the late endosomal/lysosomal compartment a series of proteolytic and N-glycan processing events to yield a mature active form composed of four tightly associated peptides19,20.

What does acid alpha-glucosidase do?

Acid alpha-glucosidase, also called α-1,4-glucosidase and acid maltase, is an enzyme (EC 3.2. 1.20) that helps to break down glycogen in the lysosome.

How do you make alpha-glucosidase?

α-Glucosidase Enzyme Solution (Immediately before use, prepare a solution containing 0.15-0.3 unit/mL of α- Glucosidase in cold deionized water.) Mix by inversion and transfer the solutions to suitable cuvettes. Record the A400nm for both the Test and Blank using a suitable spectrophotometer.

Is alpha amylase and alpha glucosidase?

Alpha glucosidase and alpha amylase are the important enzymes involved in the digestion of carbohydrates. Alpha Amylase is involved in the breakdown of long chain carbohydrates and alpha glucosidase breaks down starch and disaccharides to glucose.

What kind of macromolecule is alpha glucosidase?

Alpha-glucosidase is a carbohydrate-hydrolase that releases alpha-glucose as opposed to beta-glucose. Beta-glucose residues can be released by glucoamylase, a functionally similar enzyme.

Is acid alpha glucosidase an enzyme?

Acid alpha-glucosidase is the enzyme responsible for degradation of glycogen polymers to glucose in the acidic milieu of the lysosomes. Cardiac and skeletal muscles are the two major tissues affected by the accumulation of glycogen within the lysosomes.

What molecule does acid alpha glucosidase break down?

Lysosomes use digestive enzymes to break down complex molecules into simpler ones that can be used by cells. Acid alpha-glucosidase normally breaks down a complex sugar called glycogen into a simpler sugar called glucose. Glucose is the main energy source for most cells.

Is acid alpha-glucosidase an enzyme?

Is McArdle disease fatal?

The disease can lead to dark urine. Severe, uncontrolled McArdle disease can cause life-threatening kidney problems. You can work with your care team to make a diet and exercise plan that helps you control McArdle disease and its complications.

What is alpha amylase and alpha glucosidase?

What is alpha glucosidase inhibitory activity?

𝛼 -glucosidase plays a role in the conversion of carbohydrates into glucose. By inhibiting 𝛼 -glucosidase, glucose levels in the blood can be returned within normal limits [5.

What is the function of acid alpha glucosidase?

Errors in this gene cause glycogen storage disease type II (Pompe disease). This gene encodes lysosomal alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing.

How big is the gene coding for alpha glucosidase?

The gene coding for human lysosomal alpha-glucosidase is about 20 kb long and its structure has been cloned and confirmed. Human lysosomal alpha-glucosidase has been studied for the significance of the Asp-518 and other residues in proximity of the enzyme’s active site.

Are there any other enzymes similar to alpha glucosidase?

Alpha-glucosidase breaks down starch and disaccharides to glucose. Maltase, a similar enzyme that cleaves maltose, is nearly functionally equivalent. Other glucosidases include: Cellulase. Beta-glucosidase. Debranching enzyme.

What is the role of asp-518 in alpha glucosidase?

Human lysosomal alpha-glucosidase has been studied for the significance of the Asp-518 and other residues in proximity of the enzyme’s active site. It was found that substituting Asp-513 with Glu-513 interferes with posttranslational modification and intracellular transport of alpha-glucosidase’s precursor.