Contents
Is palmitic acid soluble in water?
Palmitic acid
| Names | |
|---|---|
| Solubility in water | 0.46 mg/L (0 °C) 0.719 mg/L (20 °C) 0.826 mg/L (30 °C) 0.99 mg/L (45 °C) 1.18 mg/L (60 °C) |
| Solubility | Soluble in amyl acetate, alcohol, CCl4, C6H6 Very soluble in CHCl3 |
| Solubility in ethanol | 2 g/100 mL (0 °C) 2.8 g/100 mL (10 °C) 9.2 g/100 mL (20 °C) 31.9 g/100 mL (40 °C) |
Is palmitic acid soluble in hexane?
The solubility of palmitic acid has been measured in ethanol, 2-propanol, acetone, heptane, hexane, and trichloroethylene and in the azeotropic mixtures of the solvents (ethanol-heptane; hexane-ethanol; ethanol-trichloroethylene; acetone-heptane; heptane-2-propanol; acetone-hexane; hexane-2-propanol; 2-propanol- …
How do you dissolve palmitate?
If you would like to avoid interference of your bacteria with solvents, prepare 100 mM stock solution of palmitic acid. First dissolve sufficient amount of NaOH (about 3.5 g per 100 mL) then add palmitic acid and place it in a water bath about 70-80oC. Wait for dissolution.
How do you dissolve palmitic acid for cell culture?
Palmitic can be dissolved at 10 mmol/l in M-199 medium containing 11% fatty acid–free bovine serum albumin (BSA) (Sigma) under N2 atmosphere, shaken overnight at 37°C, sonicated for 15 min, and filtrated under sterile conditions (stock solution).
What is palmitic acid soluble in?
Palmitic acid is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of palmitic acid in ethanol is approximately 30 mg/ml and approximately 20 mg/ml in DMSO and DMF. Palmitic acid is sparingly soluble in aqueous buffers.
How do you make palmitic acid solution?
This one down is pretty handy.
- Dissolve 0.1282g of PA in 1 mL (500 mM) of 100% ethanol and heat at 70°C.
- Make 10% BSA in cell culture media (DMEM, KSF, etc.),
- Add 10 uL of dissolved PA into 990 uL of 10% BSA.
- Vortex and incubate at 55°C for 15 min.
- Repeat the step 4.
- Use 10 uL of ethanol in 990 uL of 10% BSA to make control.
Is palmitic acid soluble in ethanol?
Is palmitic acid hydrophobic?
The carboxylic acid is hydrophilic. Lastly, palmitic acid ( ) is definitely an amphipathic molecule. The carboxylic acid portion is hydrophilic, and the hydrocarbon chain is very long, making up the hydrophobic region.
Does palmitic acid dissolve in ethanol?
Is palmitate palmitic acid?
Palmitate (more formally known as retinyl palmitate) contains palmitic acid, a 16-carbon saturated fatty acid, which is the major fatty acid found in palm oil. The palmitic acid is attached to the alcohol form of vitamin A, called retinol, to make vitamin A stable in milk.
How do you dilute palmitic acid?
Palmitic acid is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, palmitic acid should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. Palmitic acid has a solubility of approximately 0.25 mg/ml in a 1:2 solution of ethanol:PBS (pH 7.2) using this method.
What is the formula of palmitic acid?
C16H32O2
Palmitic acid/Formula
What is the chemical formula for palmitic acid?
Palmitic acid PubChem CID 985 Structure Find Similar Structures Chemical Safety Laboratory Chemical Safety Summary (LCSS Molecular Formula C16H32O2 Synonyms palmitic acid Hexadecanoic acid 57-10-3
Which is the best way to dissolve palmitic acid?
The best way to dissolve palmitic acid is by dissolving it in a fatty acid free medium, containing fatty acid free-BSA. As BSA has 11 fatty acid binding sites, the maximal molar FA/albumin ratio that can be used is 11 but in order to be on the safe side we use a ratio around 8, and this is the ratio that Sandip suggested.
How does methanol affect solubility of palmitic acid?
Though the solubility of palmitic acid is more in mixture of hexane with alcohols as compared to pure solvents [44], it displaces the palmitic acid from the active acid sites on the surface of the catalyst and thus lowers its interaction with methanol resulting into decreased yield of methyl palmitate. Liu et al.
Which is the best catalyst for palmitic acid esterification?
Sulfated zirconia (SZr) solid acid catalyst, prepared by conventional precipitation technique was studied for esterification of palmitic acid with methanol to synthesize methyl palmitate. The catalyst exhibited 90% yield of methyl palmitate at 60°C over 6 wt% catalyst.