Contents
What is trnL trnF?
The trnL-trnF region is located in the large single-copy region of the chloroplast genome. It consists of the trnL gene, a group I intron, and the trnL-F intergenic spacer.
What is trnL gene?
The trnL gene is part of trnL-F region of chloroplast genome that split by group I intron, the intergenic spacer and trnF exons (Figure 1) and is co-transcribed (Bakker et al. 2000). The intron is positioned between the U and the A of the UAA anticodon loop.
What is its region in fungi?
The ITS region is the most widely sequenced DNA region in molecular ecology of fungi and has been recommended as the universal fungal barcode sequence. It has typically been most useful for molecular systematics at the species to genus level, and even within species (e.g., to identify geographic races).
What is rbcL marker?
The rbcL marker based method was adopted to identify green isolates from monospecies but non-axenic cultures of microalgae with 18S universal markers as control. In present study, authors have developed and validated a novel method for molecular identification of microalgae even from non- axenic cultures.
What is its primer?
From Wikipedia, the free encyclopedia. Internal transcribed spacer (ITS) is the spacer DNA situated between the small-subunit ribosomal RNA (rRNA) and large-subunit rRNA genes in the chromosome or the corresponding transcribed region in the polycistronic rRNA precursor transcript.
Why do you think the its regions are used for fungal identification?
The ITS region is a useful barcode marker because it usually can be sequenced from previously described fungi for which no sequence data are currently available by sequencing the type material (i.e., the specimen on which a species is originally described and deposited in a fungarium).
What does the L stand for in rbcL?
Acronym. Definition. RbcL. Ribulose Bisphosphate Carboxylase Large (microbiology)
What is rbcL used for?
Within angiosperms the rbcL gene has been widely sequenced and used for inferring plant phylogenies at higher taxonomic levels. Unfortunately rbcL does not usually contain enough information to resolve relationships between closely related genera, such as Hordeum, Triticum, and Aegilops.
What is the difference between 18S and its?
Similar to 18S rRNA, ITS is often used in metagenomic analysis. However, 18S rRNA is mainly used for high resolution taxonomic studies of fungi, while the ITS region is mainly used for fungal diversity studies as a fungal barcode marker.
What is ITS1 and ITS4?
ITS1 and ITS4 are general primers that amplifies the Internal Transcribed Spacer region for identification purpose. You can easily find a journal that provides the sequences for these primers: https://benthamopen.com/FULLTEXT/TOBIOTJ-14-70. Cite.
What is ITS primer?
How do you identify a fungus?
Fungi are identified by their morphology in culture. Fungi have mycelium and spores which are used in the identification. Therefore you have to search for mycelium (hyphae), the spores, origin of the spores, asexual or sexual; and their structure and morphology. So you have to see the morphology clearly.
Why are rbcL and trnL-F used in PCR?
PCR-protocols were robust. Sequencing success was >95% for rbcL, and equally high for trnL-F when using internal primers. The slightly higher effort needed for trnL-F in some taxa (mainly belonging to the families Blechnaceae and Thelypteridaceae) resulted from the presence of a homopolymer C-repeat in the intergenic spacer.
How many base pairs are in the trnL intron?
Seven hundred fifty-two to one thousand ninety-seven base pairs of the trnL intron and trnL- trnF intergenic spacer of the chloroplast DNA of 55 Juncaceae taxa (Juncus, Luzula, Rostkovia, and Oxychloë) was sequenced.
How are rbcL and trnL-F used to identify fern species?
Our results suggest a high potential of the combined use of rbcL and trnL-F as a two-locus cpDNA barcode for identification of fern species. A regional approach may be preferred for ecological tests.
Why are trnL intron and intergenic spacer important?
The structural mutations in the trnL intron and the trnL- trnF intergenic spacer are useful for phylogenetic reconstruction in the Juncaceae. Base Pairing Base Sequence DNA Primers