Contents
What is rapid amplification of cDNA ends used for?
Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell.
Can cDNA be amplified by PCR?
Amplification of the desired portion of cDNA can be achieved in PCRs primed, for example, by sense and antisense oligonucleotide primers corresponding to specific sequences in particular cDNAs. For maximum specificity, the antisense primer should be located upstream of the oligonucleotide used to prime cDNA synthesis.
What is the purpose of race PCR?
Rapid amplification of cDNA ends (RACE) is a technique used to obtain the full-length sequences of RNA transcripts. RACE can provide the sequence of an RNA transcript from a short, known sequence within the transcript all the way to the 5′ end (5′ RACE-PCR) or 3′ end (3′ RACE-PCR) of the RNA.
What is the purpose of 5 race?
The 5′ RACE System is a set of prequalified reagents intended for synthesis of first strand cDNA, purification of first strand products, homopolymeric tailing, and preparation of target cDNA for subsequent amplification by PCR.
What is RDT race?
Rapid Amplification of cDNA Ends (RACE) is a procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and unknown sequences at either the 3′ or the 5′ -end of the mRNA (1).
Is cDNA amplified?
First-strand cDNAs are synthesized using a special hairpin structure oligo(dT)-anchor primer and the core sequences of target gene are amplified by up-down PCR (step 1).
Can you amplify cDNA?
It requires only minimal amounts of total RNA for the synthesis of first-strand cDNA, while the same cDNA can be used to amplify flanking sequences of any cDNA species present in the sample. This method is reliable and easy to perform, which is very useful for isolating cDNA species of rare transcripts.
How does cDNA synthesis work?
The synthesis of DNA from an RNA template, via reverse transcription, results in complementary DNA (cDNA). cDNA can then serve as template in a variety of downstream applications for RNA studies such as gene expression; therefore, cDNA synthesis is the first step for many protocols in molecular biology.
How are cDNA ends amplified?
Specific cDNA is then directly amplified by PCR using a gene-specific primer (GSP) that anneals to a region of known exon sequences and an adapter primer that targets the poly(A) tail region. This permits the capture of unknown 3′-mRNA sequences that lie between the exon and the poly(A) tail.
What is the second primer in case of 5 race?
During PCR, the thermostable DNA polymerase is directed to the appropriate target RNA by a single primer derived from the region of known sequence; the second primer required for PCR is complementary to a general feature of the target-in the case of 5′ RACE, to a homopolymeric tail added (via terminal transferase) to …
What are the 5 different races?
OMB requires that race data be collectd for a minimum of five groups: White, Black or African American, American Indian or Alaska Native, Asian, and Native Hawaiian or Other Pacific Islander. OMB permits the Census Bureau to also use a sixth category – Some Other Race. Respondents may report more than one race.
Which is the race system for cDNA amplification?
Product overview. Description. 3´ RACE System is suitable for rapid amplification of cDNA ends (RACE) (1-3) and anchored PCR between a defined point within mRNA and the 3´ poly(A) end (Figure 1).
How is the 3’race system for rapid amplification?
Learn more 3´ RACE System is suitable for rapid amplification of cDNA ends (RACE) (1-3) and anchored PCR between a defined point within mRNA and the 3´ poly (A) end (Figure 1). The system is useful for the amplification of rare messages for which little sequence information is available, and for capturing the 3´ end information of mRNA.
How is the 3’race system used in PCR?
3´ RACE System is suitable for rapid amplification of cDNA ends (RACE) (1-3) and anchored PCR between a defined point within mRNA and the 3´ poly (A) end (Figure 1). The system is useful for the amplification of rare messages for which little sequence information is available, and for capturing the 3´ end information of mRNA. The 3´ RACE System:
How are mRNAs converted to cDNA using 3’race?
3′ RACE takes advantage of the natural poly (A) tail found in mRNA as a generic priming site for PCR. In this procedure, mRNAs are converted into cDNA using reverse transcriptase (RT) and an oligo-dT adapter primer.