Contents
What does BP mean in DNA ladder?
Introduction. DNA molecular weight (mw) standard controls of nucleic acids, also known as DNA ladders, are widely used in molecular biology studies to determine the mw or the base pair (bp) length of nucleic acids. The DNA ladder is also used to quantitatively analyze target DNA fragments.
How much DNA ladder should I load?
For a standard electrophoresis system, we recommend loading 0.5 µg (20 µl) of the Fast DNA Ladder on the agarose gel. For a fast electrophoresis system (5 to 30 minutes separation), follow the system’s manufacturer recommendations: 5 to 20 µl load. A dilution of the ladder may be required.
How do you dilute a 1kb ladder?
If you want to prepare a working stock of the 1 kb ladder with loading dye, measure amount of ladder in the tube and add the loading dye accordingly. Use water to dilute your loading dye, in the ratio of 1 part ladder, 1 part buffer, and 4 parts ddH20 (if your loading buffer is 6X).
What is bp DNA?
A base pair (bp) is a fundamental unit of double-stranded nucleic acids consisting of two nucleobases bound to each other by hydrogen bonds. They form the building blocks of the DNA double helix and contribute to the folded structure of both DNA and RNA.
What is a 50 bp ladder?
Description: 50 bp DNA Ladder consists of 10 DNA fragments ranging from 50 bp to 500 bp and is supplied in ready-to-use format containing an orange tracking dye. It allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest.
How much loading dye do you put on a ladder?
The recommended loading volume of the 1 kb DNA ladder is 6 μL per lane. DNA Loading Dye is a ready-to-use marker dye containing orange G (0.4%), bromophenol blue (0.03%), and xylene cyanol FF (0.03%), in 15% Ficoll® 400, 10 mM Tris-HCl (pH 7.5), and 50 mM EDTA (pH 8.0).
How do you dilute 6X DNA loading dye?
6X Universal & Glow Dyes Dilute one part 6X Dye solution into five parts of sample solution to give a final concentration of 1X Dye solution. The sample is then ready to load to a gel. For Example: 10µl sample and 2µl 6X Dye Solution.
What is a 100 bp ladder?
Invitrogen 100 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 2,000 bp. 100 bp DNA Ladder consists of 13 individual chromatography-purified DNA fragments and has reference bands at 2000, 1500, and 600 bp for easy orientation.
What does 1 kb ladder mean?
Product Description. 1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 – 10,000 base pairs (bp). The ladder consists of 13 double-stranded, blunt-end fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1).
Is it possible for you to synthesize 100 bp ladder in our laboratory?
For instance, labeled 100 bp DNA ladder can be produced by using labeled dNTPs in the PCR amplification. In fact, we have successfully produced the 60 bp DNA ladder and the 500 bp DNA ladder supplied for our specific needs (Figure 3).
Where on the gel will the largest DNA molecules be and why?
The largest fragments are near the top of the gel (negative electrode, where they began), and the smallest fragments are near the bottom (positive electrode).
Which is the 1000 bp / 1 kb DNA marker?
DNA Marker 1000 bp / 1 kb is designed for use as molecular weight standard for agarose gel electrophoresis. Features: 1000 bp/1 kb DNA Marker from GeneON is composed of 13 individual DNA fragments which are re-dissolved in storage buffer. Description/Preparation: The 1000 bp/1 kb DNA Ladder has a number of proprietary plasmids which are…
How many fragments are in the 100 bp DNA ladder?
The 100 bp DNA Ladder consists of 15 blunt-ended fragments between 100 and 1500 bp in multiples of 100 bp and an additional fragment at 2072 bp. The 600 bp band is approximately 2 to 3 times brighter than the other ladder bands to provide internal orientation. This ladder is not designed for quantitation.
What are the colors of the spectra protein ladder?
Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color (blue, orange, green, pink) protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and western blotting.